Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLSLKSLQCFVTLVKTKSFTRTAEELYLTQPTISKILQQLEEQLQVQLLVKPDHGRKRQIELTEIGERIYQHAGELLQAEQNIFLEIENYQQLKTGTLKLGVPPL-GSQLLTTALFDFHRQWPDIELAFMEVGSRGIEQALLNNELDVGVLLQPF--DEQTFNSIELCNYPLMVLLRRDATWATRKKINLEELQHQSFLMFPENFSLNSIILDACKQHGF-YPTIACRTSQWHLLADMVLQRMGIALLPQYYTDM--LDPTLFAAVPLEKPNIQWHLVMAWKKNLPVSPAVQAWLSIVRQHFQHIKP 3FXQ Chain:A ((1-296)) MLKLQTLQALICIEEVGSLRAAAQLLHLSQPALSAAIQQLEDELKAPLLVRT----KRGVSLTSFGQAFMKHARLIVTESRRAQEEIGQLRGRWEGHITFAASPAIALAALPLALASFAREFPDVTVNVRDGMYPAVSPQLRDGTLDFALTAAHKHDIDTDLEAQPLYVSDVVIVGQRQHPMANA--TRLAELQECRWAFSSAPRGPGAIIRNAFARYGLPEPKLGLVCESFLALPGVVAHSDLLTTMPRTLYERNAFKD-QLCSIPLQDALPNPTIYVLRRHDLPVTPAAAGLIRWIQHHAL----

General information: TITO was launched using: RESULT: Template: 3FXQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1297 -86309 -66.55 -297.62 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -66.55 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_3FXQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FXQ-query.scw PDB file : Tito_Scwrl_3FXQ.pdb: