Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHRQVLILASSQSLFQTVSVMVMTIGGLAGAN-IANTPTLSTLPIASMFLGTALMMFPASILMAHIGRRNGFLFG---AFLGVLGGIIASIGIFYSSL-SLLALGTLCVGAYQSFAQFY-RFAASEVAND--AFRSRAISWVLAGGIVAALIGPTLARFGGPLFQHLEYIGSFLIISIISLVAMGILSSLHIADT-VE-QK-SD-F----TAGRPWQQIVFQPTYLVALFGAITGYGIMILGMTATPIAMRH-S---HHELGSITTVIQLHVLGMFLPSFFTGNLIARFGVLKIMFAGLLLFACYIGFALSGIQFTSFAISLILLGVGWNFLFIGSTSLLTGTYSVAEKAKAQAINDMTV-FVVGLICSFSAGALLDLFGWKAMNIALIPWLVIAAASLFWLSQKREKQFV 1PV7 Chain:A ((6-397)) -NTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSDTGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFI---FGPLLQYNILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALGASIVGIMFT----INNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFRQPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIMFFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCFKYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGFTLISV-----------

General information: TITO was launched using: RESULT: Template: 1PV7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1679 -248324 -147.90 -669.34 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -147.90 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.304

(partial model without unconserved sides chains): PDB file : Tito_1PV7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PV7-query.scw PDB file : Tito_Scwrl_1PV7.pdb: