Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIGQYRVTALIPARGGSKRLPRKNVKLLVDKPLIAWSIEVAKASKYIDRVVVSTDDEEIKQVSEQYGAEVPFLRPEYLSNDHASSFDVIKHAIDFLHLGQKNELIVLLQPTSPLRLVSELDTALEFFIAKNAKGIVSISETEHSPMWSN--------TLPENGCMSDFIRPEVQGKRSQDLPKFFRLNGSIYIYETLCLLEQSKIFFNENVYGFETSLKTAIDIDTDLDFLIAETIMKNRAIE 1QWJ Chain:D ((4-217)) ------LAALVLARGGSKGIPLKNIKRLAGVPLIGWVLRAALDAGVFQSVWVSTDHDEIENVAKQFGAQV-HRRSSETSKDSSTSLDAI---VEFLNYHNEVDIVGNIQATSPCLHPTDLQKVAEMIREEGYDSVFSVVR-RHQFRWSEIQKGVREVTEPLN--LNPAKRP-----RRQDWDGELYENGSFY-FAKRHLIEMGYLQGGKMAY-YEMRAEHSVDIDVDIDWPIAE---------

General information: TITO was launched using: RESULT: Template: 1QWJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1001 -84135 -84.05 -408.42 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain D : 0.74 3D Compatibility (PKB) : -84.05 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.424

(partial model without unconserved sides chains): PDB file : Tito_1QWJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QWJ-query.scw PDB file : Tito_Scwrl_1QWJ.pdb: