Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSKGFTLLEVMVALAIFAVAAVALTKVAMQYTQSTSNAILRTKAQFVAMNEVAMMEINQEWLQ-GTQSKQVTSQGETWQIDKSAQSTISPNVQKIDLQVSLYDLDKGK--VQ-NGITHMVFFNYPVKAK 5BW0 Chain:F ((4-93)) ----------------------------------NASRLEDKTLAMWIADNRLNELQLEQTPPSSGRNQGELEFAGRRWEWRTQVDSTAEQDMRRVIVWVAAKP-GR--GSIEERAAARLVGFL------

General information: TITO was launched using: RESULT: Template: 5BW0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 301 3081 10.23 37.11 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain F : 0.61 3D Compatibility (PKB) : 10.23 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.333

(partial model without unconserved sides chains): PDB file : Tito_5BW0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5BW0-query.scw PDB file : Tito_Scwrl_5BW0.pdb: