Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQPTTPSQSVIPAWVDSSLLQGMLRGIERESLRMQSNGFLSQELHPKALGSAL---TH-PKITTDYSEALMEFITSPQPTIGDALHELTDIHAVVHRHLE-NGEKLWPLSMPCTLDDNEERIRLAQYGTSNIGRFKTLYRRGLGIRYG--RRMQTISGVHYNLSFPDSLFSALQAHETDEKLKALSLQDYRSHRYFGLIRNFIRLTPLVMFLVGASPSVCHCFLTGHEHHLLPLIKGSYYLPYATALRMGRLGYQNSAQKSLGIHYNNLTDYLDGLQKAVHSPYPPFSRLGLNDASGEPLQINDHVLQIENEYYSLVRPKQVPQAGETPSQALKNRGVGYVELRAVDVNPYSAIGVNETTAGFLEALALYCLLSDSPELLCPEQDLIEKNQTEVVNRGRAPNATITDLNGSYHIEDWARQHISKMQDCARLLDQTYATDLYSSALAVMQERIDEVDETLSAHVIDDTLKHGGTWSFGSHMAQLHAETYEKHEISAETLQYFEQLAVQSLQQQEQLEQDSQISFDQYLEQYR 1R8G Chain:B ((8-355)) -------------------VSEPFTLGIELEMQVVNPPGYDL-SQDSSMLIDAVKNKITAGEVKH-ITESMLELATDVCRDINQAAGQFSAMQKVVLQAATDHHLEICGGGTHPFQKWQ---------------------QR-TLENFGYLIQQATVFGQHVHVGCASG----------------D--------DAIYLLHGLSRFVPHFIALSAASPYMQGTD-TRF-----------------ASSRPNIFSAFPD--NGPMPWVSNWQQFEALFRCLSYTT----------------------MIDSIKDLHWDIRPSPHF---------------GTVEVRVMDTPL-TLSHA-VNMAGLIQATAHWLLTERPFKHQEKDYLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGAS-------SAIEALHRQVVS-GLNEAQLMRDFVADG-------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1R8G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1814 -34465 -19.00 -104.44 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -19.00 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.338

(partial model without unconserved sides chains): PDB file : Tito_1R8G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1R8G-query.scw PDB file : Tito_Scwrl_1R8G.pdb: