Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVRTRIAPSPTGFPHVGTAYIALFNMCFAKQHGGEFILRIEDTDQLRSTPESEKMILDSLRWLGLNWSEGPDVGGPHAPYRQSERMGIYKQYALELVEKGHAFYCFATAEELDQMRAEQQARGETPKYDGRGLKLSQEEVARRLEAGEPHVIRMKVPEEGVCKFNDLLRGEVEIPWAQVDMQVLLKTDGLPTYHLANVVDDHLMEITHVLRGEEWLPSAPKHQLLYQYFGWEMPTLCHMPLLRNPDKSKLSKRKNPTSINYYRDIGVLPEALLNYLGRMGWSMPDEREVFTLQDMMDNFDIQRVSLGGPIFDVEKLNWLNGQWIKGLTPGQLLDRLLTWKSDRSTLEDIAAAIQPRINLLSEAVNWAGFYFNHMPQITAEMFESKKLTQEQVRQSLQFAIWRLESQFTWNNDTVGQTLMDLANQMGIKLRDFMPTFFIAIAGSTSSTPVMQSMVTLGPDLTFARLRHALEIVGAPSKKEVKNWEKLNESLKLPKNEATSEA 5TGT Chain:B ((12-500)) --VRTRIAPSPTGDPHVGTAYIALFNLCFARQHGGQFILRIED-----STRESEQQIYDALRWLGIEWDEGPDVGGPHGPYRQSERGHIYKKYSDELVEKGHAFTCF--------------------------MHLPKDEVQRRLAAGESHVTRMKVPTEGVCVVPDMLRGDVEIPWDRMDMQVLMKADGLPTYFLANVVDDHLMGITHVLRGEEWLPSAPKLIKLYEYFGWEQPQLCYMPLLRNPDKSKLSKRKNPTSITFYERMGYLPQALLNYLGRMGW-------KFTLAEMIEHFDLSRVSLGGPIFDLEKLSWLNGQWIREQSVEEFAREVQKWALNPEYLMKIAPHVQGRVENFSQIAPLAGFFFSGGVPLDASLFEHKKLDPTQVRQVLQLVLWKLESLRQWEKERITGCIQAVAEHLQLKLRDVMPLMFPAITGHASSVSVLDAMEILGADLSRYRLRQALELLGGASKKETKEWEKIRDAI-----------

General information: TITO was launched using: RESULT: Template: 5TGT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2087 -253052 -121.25 -561.09 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.87 3D Compatibility (PKB) : -121.25 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.87 QMean score : 0.542

(partial model without unconserved sides chains): PDB file : Tito_5TGT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TGT-query.scw PDB file : Tito_Scwrl_5TGT.pdb: