Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------------------------------------MLLWLFEQLAGYHSSFQVVRYLT-LRSLLSVLTSL---TIGLVLGPIMIRKLQ-ALKYGQAVSSF-APENHAKKMGTPTMGGI---LILLSIGISTLLWADLSNPYVWIVLGVMVVFGAVGWADDWIKIRYKDNAGLPARKKFFWTSVASLGAGIA---LYLIATQQSNAEYTANMLDLLIPFFKNLSIP---LSIV--PLGLAFIVFTYLVI-----NGASNAVNLTDGLDGLAIMPVVMVATGLGVFAYLSGDIR------FANYLHIPYVKYTSELVVICSAMIGAGLAFLWYNAHPAQVFMGDVGAL--ALGAMLGTIAVMVRQEIVF--AIMGGVFVMEAVSVFLQIGSLRMRNKRVFLMAPLHHHYEKQGWKETQVVIRFWIITIMLV--VLGLMTLKLR 5MSX Chain:A ((21-459)) HNNPFGNALIPDMIADASIQEINGVFYCYATTDGYGQGLKTSGPPVVWKSKDFVHWSFDG--TYFPSAAKEKYWAPSKAIFANGKYYIYPTINGYMYPAVADKPEGPFKLARGKDEFYKPFTPSTLLQSKNPGGIDAEIFVDDDGQAYVFWG---RRHVAKLNEDMITVDSVVQVISTPRKEYSEGPIFFKRKGIYYY-LYTIGGDEKYQYAYVMSRVSPMGPFEAPEQDIISTTNYERGIFGPGHGCVFHPEGTDNYYFAYLEFGRRSTNRQTYVNQLKFNEDG-TIRPVELTMDGVGALKKVKSDKKMKIDTVYASSIEVP-LKIEPMKDPTCLRTEYFVPSFAVDGANGSRWMAAAEDSINPWIVADLGTVKKVRRSEIYFVRPTAGHAYVIEASM-----------DGKVWQEFAVHQDRKMCSPHTDVLNKRFRYLRIKILKGVPGIWEWNIY

General information: TITO was launched using: RESULT: Template: 5MSX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2037 -89482 -43.93 -253.49 target 2D structure prediction score : 0.21 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -43.93 2D Compatibility (Sec. Struct. Predict.) : 0.21 1D Compatibility (Hydrophobicity) : 0.64 QMean score : -0.008

(partial model without unconserved sides chains): PDB file : Tito_5MSX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MSX-query.scw PDB file : Tito_Scwrl_5MSX.pdb: