Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGHSVALNFADGKTFFISVNKDELLLDAAVRQGINLPLDCREGVCGTCQGQCETGIY---EQEYVDEDALSERDLAERKMLACQTRVKSDAAFYFDHDSAICNAGDTLKIETQVTAVELVSETTAILHLDASNHAEQLQFLPGQYARLQIPDTEDWRSYSFANRPNATNQLQ-FLIRLLPDGVMSNYLRDRCQVGQSLLIEAPLGSFYLREVERPLVFVAGGTGLSAFLGMLDNLVEQPNSPTVQFYYGVNSETDLCEQQRLHAYAEQLPNFSYHPIVTKATETWQGKAGYIHEHLNKDQLAEQAFDMYLCGPPPMIEAVKNWLDEQALQNYRIYSEKFLQSNTSR 1KRH Chain:B ((4-335)) --HQVALQFEDGVTRFICIAQGETLSDAAYRQQINIPMDCREGECGTCRAFCESGNYDMPEDNYI-EDALTPEEAQQGYVLACQCRPTSDAVFQIQASSEVCKT-KIHHFEGTLARVENLSDSTITFDIQLDDGQPDIHFLAGQYVNVTLPGTTETRSYSFSSQPG--NRLTGFVVRNVPQGKMSEYLSVQAKAGDKMSFTGPFGSFYLRDVKRPVLMLAGGTGIAPFLSMLQVLEQKGSEHPVRLVFGVTQDCDLVALEQLDALQQKLPWFEYRTVVAHA-ESQHERKGYVTGHIEYDWLNGGEVDVYLCGPVPMVEAVRSWLDTQGIQPANFLFEKF-------

General information: TITO was launched using: RESULT: Template: 1KRH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1849 -37031 -20.03 -112.90 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -20.03 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.388

(partial model without unconserved sides chains): PDB file : Tito_1KRH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KRH-query.scw PDB file : Tito_Scwrl_1KRH.pdb: