Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHIYILGSAAGGGCPQWNCNCPNCHGVRTGTINAKVRTQSSIAISENGVDWILLNASPDIRQQLFDFKAAQPARKLRDTGITNVILMDSQLDHTTGLLTLREGCPMNVWCTEMVYQDLTTGFPVFNMLKHWNGGLQYHQVDPKQAFIIDGFENLEFLPLIIQSAAPPYSPHRHDPHEGDNIALIIKDHKTQKQLFYAPGLGKIDDQIMQIMQDSDCVMIDGTLWTDDEMQQTGVGKKTGREMGHLYISGEGGSLSYLNQLSTPKKVLIHINNTNPILNEDSAQFAELKANGVEVAFDGMQIEL 4Z7R Chain:B ((1-299)) MHVVILGSAAGGGVPQWNCRCSICSLAWAGDSRVRPRTQSSIAVSPDGERWLLLNASPDIRQQIQANPQMHPREGLRHSPIHAVLLTNGDVDHVAGLLTLREGQPFTLYATPGILASVSDNR-VFDVM--AADVVKRQTIALNETFEP--VPGLSVTLFSVP-----------------TVGTMIEAG--GKRLAYIPGCARVTEDLKARIAGADALLFDGTVLEDDDMIRAGVGTKTGWRMGHIQMNGETGSIASLADIEIGRRVFVHINNTNPVLIEDSYERASVEARGWTVAHDGLTLDL

General information: TITO was launched using: RESULT: Template: 4Z7R.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1706 -208912 -122.46 -748.79 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -122.46 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.434

(partial model without unconserved sides chains): PDB file : Tito_4Z7R.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Z7R-query.scw PDB file : Tito_Scwrl_4Z7R.pdb: