Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKIYRLSMSLIANTTVVRGRFLDIQQTVSEPTDIPNQVRYLEDGVLISEHGKIKWFGAWEDAQQHLP---AGVEVQHYPEQLIVPGFIDTHIHFPQTEMVG-AYGEQLLSWLNTYTFPTEIQFQDKTYASEIAQFFVQELLKHGTTTALVFCTVHPESVDALFEAAERVQMRLIAGKVLMDRNAPEALCDTPETAYSNTKALIEKWHGKGRALY-AITPRFAPTSTPEQLERAGQLKQEFPDVYVHTHLSENKDEIAWVKSLFPEQAGYLDVYQHYGLTGKR-SVFAHCVHLEDEEWQCMHDTQSAIAFCPTSNLFLGSGLFPLKKTWEKQVKVGLGTDIGAGTSFSLLQTVNEAYKVQ------QLQGDK---LSAYEALYHATLGGAKALDLQDQLGNFNIGKEADFVVLNLKPTALQELRQTKSKSVEDSLFALFTLGDDRNIEATYIYGNRAYQKETAK 2I9U Chain:B ((20-427)) -------------------------------TKTSDKFTIMKDSYIVVIDGKI------ASVSSNLPDKYKGNPIIDFRNNIIIPGMNDLHAHASQYKNLGIGMDKELLPWLNNYTFPEEAKFLNVDYAKKTYGRLIKDLIKNGTTRVALFATLHKDSTIELFNMLIKSGIGAYVGKVNMDYNCPDYLTENYITSLNDTEEIILKYKDKSNIVKPIITPRFVPSCSNELMDGLGKLSYKYR-LPVQSHLSENLDEIAVVKSLHKKSNFYGEVYDKFGLFGNTPTLMAHCIHSSKEEINLIKRNNVTIVHCPTSNFNLGSGMMPVRKYLNLGINVVLGSDISAGHTCSLFKVI--AYAIQNSKIKWQESGKKDMFLSTSEAFYMATKKGGSFF---GKVGSFEEGYDFDALVINDSNLYPEDYDLT------ERLERFIYLGDDRNIMKRYVCGNEIF------

General information: TITO was launched using: RESULT: Template: 2I9U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2334 20741 8.89 52.77 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : 8.89 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.478

(partial model without unconserved sides chains): PDB file : Tito_2I9U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I9U-query.scw PDB file : Tito_Scwrl_2I9U.pdb: