Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKLLSFLKNKALGSSIDYKILPRKVKFDWKDTPIDWIPNQPFASYFINEINNILPAGEFWFCRLYNKVLPRITDEKLKQDVQAFIRQEAMHANAHTSANKEYLSARNIDIQRNL-DIMNY-----LFTTALADKPFDKEVPQFLQEQWDLFRLGVIATVEHMTCVLGKYALYNKRWE-ELGADPEMVDLVKWHGSEEIEHRTVAFDLYRHLGGGYIPRYYLSLAVIVLVLGLWVDGAAHIMKQDPRFADAAKSRFFAAWVALEWYKISRKDNQVLPNPIWLIAQQIDYLMPWYDPVKEGSTEDAVSYLSQSPAAKRAELQAA 3MJO Chain:B ((87-216)) --------------------------------------------------------------------LLPDAETMHEEAVYTNIAFMESVHAKSYSNIFMTLAS-T----P-QINEAFRWSEENENLQRKAK--IIMSYY---NGDDPLKKKV-ASTLLESFLFYSGFYLPMY--LSSR-AKLTNTADIIRLIIRDESVHGYYIGYKYQQGV--------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3MJO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 289 -27035 -93.55 -219.80 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.54 3D Compatibility (PKB) : -93.55 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.189

(partial model without unconserved sides chains): PDB file : Tito_3MJO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3MJO-query.scw PDB file : Tito_Scwrl_3MJO.pdb: