Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLLQQLAIKHPIFLAPMAGVSTPELAAEVSNQGGLGSLGLGANTPQSAREQILKTQALTENPFQVNFFCHQSTELNVEKAKQWLEYLRPHFEKFGAQPPQELHCIYPSFLDNDDFLNVVLETKPKTVSFHFGIPHPHQIKALKEAGILTMVSATNLIEAQAIEAAGIDIIIAQGIEAGGHRGIFN-QTFDGAIKTSDLVQLIVQHCTLPVVAAGGIMTGLQAKHMLGLGAAAVQLGTAFVQCQTSNASAEYRKALFSKPV--TQISASLSGRPARGILNHWHIKIDSPTRPVQPEYPYAYDLAKQLNALATKHQDYGFGAFWAGSNVAQIRELEAPDLVNQLVVEMLDNE 4Q4K Chain:B ((9-349)) ------LGIQQPIIQAPMLGVSTPALAAAVSNAGGLGSIAITGSAAEKGRALIREVRGLTDKPFNVNLFCHRPGQADPARERAWLDYLKPLFAEFGAEPPVRLKNIYLSFLEDPTLLPMLLEERPAAVSFHFGAPPRDQVRALQAVGIRVLVCATTPEEAALVEAAGADAVVAQGIEAGGHRGVFEPERGDAAIGTLALVRLLAARGSLPVVAAGGIMDGRGIRAALELGASAVQMGTAFVLCPESSANAAYREALKGPRAARTALTVTMSGRSARGLPNRMFFDAAAPGVPPLPDYPFVYDATKALQTAALARGNHDFAAQWAGQGAALARELPAAELLRTLVEEL----

General information: TITO was launched using: RESULT: Template: 4Q4K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2114 -131727 -62.31 -389.72 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -62.31 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.602

(partial model without unconserved sides chains): PDB file : Tito_4Q4K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Q4K-query.scw PDB file : Tito_Scwrl_4Q4K.pdb: