Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKNCGSGQSKLKALRMHIYFEVDFQEQAQHYQAVLHSRGVTVDLQPIEKLNARFLRLNPDLALCVDENGLWLSANGMKMQPDWKAEIPRLKRASLKSEMIARACQLGEKPVLVDATAGLGHDSLLMAYLGAQIQLVERHPILFTLLEDSKAQAQRDPFLSQFMDRIQLIFADSASYLKQLDQEEKTVDVVYLDPMFPQRDQNQQAIKKQAQVKKQMQLLHLLLPEDGEMDLGGHLLELAKKVAKRVIVKRPRHAIFLANQEPAHQWQGDACRFDAYFQ 1UWV Chain:A ((277-369)) ---------------------------------------------------------------------------------------------------RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGL--------QNVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGA-------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1UWV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 413 -40637 -98.39 -436.96 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -98.39 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.450

(partial model without unconserved sides chains): PDB file : Tito_1UWV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1UWV-query.scw PDB file : Tito_Scwrl_1UWV.pdb: