Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPYQPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIPAIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPYIANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLEQLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEYLSQKDLTRILDILNIQFDEE 1VZ0 Chain:A ((23-208)) ------------------------------VVRLPLASIRPNPRQPRKRFAEESLKELADSIREKGLLQPLLVRPQG-DGYELVAGERRYRAALMAGLQEVPAVVKDLTDREALELALVENLQREDLSPVEEARGYQALLEM-GLTQEEVARRVGKARSTVANALRLLQLPPEALEALERGEITAGHARALLMLE-PEDRLWGLKEILEKGLSVRQAEA-----------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1VZ0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 604 -77577 -128.44 -417.08 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : -128.44 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.709

(partial model without unconserved sides chains): PDB file : Tito_1VZ0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VZ0-query.scw PDB file : Tito_Scwrl_1VZ0.pdb: