Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------------------------TVASRRDDCWVSLFQYKANGEREAIYVDYSPPNSLVIFVHDGWRLNVQLDQHCRSLFPNDIRKQLVEGFGVHSYSKMNEVAGQKPLARSQ-------------------------------------------------------------------------------------------------- 1HUX Chain:A ((2-260)) SIYTLGIDVGSTASKCIILKDGKEIVAKSLVAVGTGTSGPARSISEVLENAHMKKEDMAFTLATGYGRNSLEGIADKQMSELSCHAMGASFIWPNVH--TVIDIGGQDVKVIHVENGTMTNFQMNDKCAAG-TGRFLDVMANILEV-KVSDLAELGAKSTKRVAISSTCTVFAESEVISQLSKGTDKIDIIAGIHRSVASRVIGLANRVGIVKDVVMTGGVAQNYGVRGALEEGLGVEIKTSPLAQYNGALGAALYAYKKAAK

General information: TITO was launched using: RESULT: Template: 1HUX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 389 4941 12.70 57.45 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : 12.70 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.364

(partial model without unconserved sides chains): PDB file : Tito_1HUX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HUX-query.scw PDB file : Tito_Scwrl_1HUX.pdb: