Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence RCTIEIRNSVGAVVGSTCITAGQPGSVQSIQSGATYTFKTSEFCGVGATN-ELIKDESLSKGGPC 2MM0 Chain:A ((1-64)) NCVANILNINEAVIATGCVPAGGELRIFVG-SSHSYLIKATSSCGLSLTNQVFINGESVQSGGRC

General information: TITO was launched using: RESULT: Template: 2MM0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 279 -21467 -76.94 -340.74 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -76.94 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.362

(partial model without unconserved sides chains): PDB file : Tito_2MM0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2MM0-query.scw PDB file : Tito_Scwrl_2MM0.pdb: