Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFSSVVELINHY 2IUI Chain:B ((13-88)) WYWGDISREEVNEKLRDTADGTFLVRDASTKMHGDYTLTLRKGGNNKLIKIFHRDGKYGFSDPLTFSSVVELINHY

General information: TITO was launched using: RESULT: Template: 2IUI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 264 -30171 -114.28 -396.99 target 2D structure prediction score : 0.86 Monomeric hydrophicity matching model chain B : 1.00 3D Compatibility (PKB) : -114.28 2D Compatibility (Sec. Struct. Predict.) : 0.86 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.788

(partial model without unconserved sides chains): PDB file : Tito_2IUI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IUI-query.scw PDB file : Tito_Scwrl_2IUI.pdb: