Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQEFINPSDGV----IRQYLATSKTLAVVGLSDREETTSNRVTKEMQARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDF----LKADAKIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA 3Q9N Chain:B ((4-140)) -------PIDGLTDEDIREILTRYKKIALVGASPKPERDANIVMKYLLEHGYDVYPVNPNYE--EVLGRKCYPSVLDIPDKVEVVDLFVN----PAKAWRFVVYAIKKGAKVVWFQYNTYYPLAA-----AG-PIIVANRCMMREHERLLG--

General information: TITO was launched using: RESULT: Template: 3Q9N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 638 10224 16.03 82.45 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : 16.03 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.566

(partial model without unconserved sides chains): PDB file : Tito_3Q9N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3Q9N-query.scw PDB file : Tito_Scwrl_3Q9N.pdb: