Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKEMSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESYASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQNETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGTSLAGQNLAYSLYQSYKSQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQHLAKVGQELSLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEGTL--LAQLYPSLEREGKAKLTAYLSSKDVARIKV----GD---------SVRYTTTHDAGNQLFLDSTITSIDATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE 1VF7 Chain:J ((26-260)) -------------------------------------------TLNTELPGRTNAF-RIAEVRPQVNGIILKRLFKEGSDVKAGQQLYQIDPATYEADYQSAQANLASTQE------------------------------------------------------------------------------QAQRYKLLVADQAVSK------------------------QQYAD-----------------------------------------------------ANAAYLQSKAAVEQARINLRYTKVLSPISGRIGRSA-VTEGALVTNGQANAMATVQQL----DPIYVDVTQPSTALLRLRRELASGQLERAGDNAAKVSLKLEDGSQ--YPLEGRLEFSEVSVDEG--TGSVTIRAVFPNP---NNELLPGMFVHAQLQ------------------

General information: TITO was launched using: RESULT: Template: 1VF7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain J - contact count / total energy / energy per contact / energy per residue : 848 -1932 -2.28 -8.78 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain J : 0.63 3D Compatibility (PKB) : -2.28 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.310

(partial model without unconserved sides chains): PDB file : Tito_1VF7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VF7-query.scw PDB file : Tito_Scwrl_1VF7.pdb: