Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLELVNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEWSKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETVEQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAYDGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGIIKEGRPYLGVIYAGLILTADG-PKVIEFNSRFGDPETQIILPRLTSDFAQNITDILDGKEPNIM--WTDKGVTLGVVVASKGYPLDYERGVELPAKTEGDVIT-----------YYAGAKFAENSRALLSNGGRVYMLVTTADTVKEAQASIYQELSQQKIEGLFYRTDIGSKAIK 5VEV Chain:B ((9-429)) MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIES-KLQNIALTAYQDLIEFCRKENIVFTVVGPEAPLAAGIVDDFRAAGLKIFGPTQYAAQLESSKDFAKAFMVKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKA--------VIVAMTLDEAHAAIDDML---------ERVVIEDFLQGEEASFIVMVDGNHVLPMATSQDHKRLLDGDKGPNTGGMGAYSPAPVVTPAVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQSGAPYTIEFNCRFGDPETQPIMSRLNSDLADLVEAAIDGRLDSVKAEWNPQ-TAVGVVLAAQNYP-------ETPKK--GDVISGLDDVNRIGKVFHAGTTVNEKGD-VLTNGGRILCVVGLGDDVAQAKAKAYGALEKISFDGMQYRKDIADKAI-

General information: TITO was launched using: RESULT: Template: 5VEV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2328 -146797 -63.06 -376.40 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -63.06 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.583

(partial model without unconserved sides chains): PDB file : Tito_5VEV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5VEV-query.scw PDB file : Tito_Scwrl_5VEV.pdb: