Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------MKAWIRQL-------------LYKIKQ----SSGAVFLCFVLKNLKFVFVSFRKIRY---TVCKRFQRSPVMAKTF--FIPNKQSILGE-QEILNAKSILALLDGLE-----SHSYDVVYLRQPLNRLE---------YIECAIVGQSQFLFK------VSYADGQKAY---RVDLPDLLTKTD---WQIIKSFLDALLAYTGTDI----------E-GLDGFDFEAYFQASIQAYLADPVARFTICQ-------GIFNPIF---------------------------------------FSRENLKSFLE--------ADGLAQFEARV---------------RAVQETDAYFARVSFYQDGEGKVHGVYHL-AQGVKTVL-PREPFVPAAYIEQLVD--KEVQ--------WEIDLVQITGDG-SKPED---YEAIARLDYAKF--------------LEVLPPSFYHQLDANQIEIQPILGQDFKTLAQEK- 4C0E Chain:A ((1674-2193)) LEAAHLLEQMEYVFDEWIHLCNNPHATERAAMIFVHQLHSVQLVTNRDEFLLFLRHALDKSVERFEQGIHSGASIAESFQAVEALVKLIIIFVKSHSAAVAFMDSILALGVLVANSHHVKRGENFNQRVFYRFFALLLHEVGLLAGHFSKSHYEQIILNFAARLFDMRPNLLPGFACAWAGLVSHRAFLPVILGLPDEKGWAPFTKLLEQFLGCVGELVKTFTVSSLGKEMYHAALKILIVLQHDFPIYLDKF--RVQLCQSLPLHATQLVNLILAAIPPNCNSLADPFQAGLKVDKIPDMKERPPTAFDSAGLLREAGLLDILERMLQNGPSEDGVAQINHAINKSTSFGYVPLGVNRRLIDAVVARFAEFAINRASSRSDSAIFVAGANDIKTLQMLVTEVSPEARYYLVSSMVNELRYPNAYTNYFSQALLDIFGHDMSDPEENLVREQIVRVLLERVLGYWPQPWGLIITILELLKNDKYLFFELPFIKATPEVAERFTALARSAA

General information: TITO was launched using: RESULT: Template: 4C0E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 21 2705 128.81 8.07 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 128.81 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.63 QMean score : ?

(partial model without unconserved sides chains): PDB file : Tito_4C0E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4C0E-query.scw PDB file : Tito_Scwrl_4C0E.pdb: