Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDD-----TPTEVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPN------------SLEIAHKRGIKIV------WTIQKDSNAPHPNTTKITVKNAH--KTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDIPGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHNVNFFK 4RQO Chain:B ((19-220)) ----MNISVFDLFS-IGIGPSSSHTVGPMLAANAFLQLLEQKNLFDKTQRVKVELYGSLALTGKGHGTDKAILNGLENKAPETVDPASMIPRMHEILDSNLLNLAGKKEIPFHEATDFLFLQKELL-PKHSNGMRFSAFDGNANLLIEQVYYSIGGGFI--TTEEDFD------NPPP-YP----FATATELLKLCKKHHLTIAELMLVNE-------------------------------------

General information: TITO was launched using: RESULT: Template: 4RQO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 791 -8459 -10.69 -50.65 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.68 3D Compatibility (PKB) : -10.69 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.354

(partial model without unconserved sides chains): PDB file : Tito_4RQO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RQO-query.scw PDB file : Tito_Scwrl_4RQO.pdb: