Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFLGLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKT-----------SIGPNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIV-GVYLSEGLPDLIRVTTVTLISLVGETAMAGAVGA-GGIGNVAIAYGFNRYNHDVTILATIVIILIIFAIQFLGDFLTKKLSHK 3D31 Chain:C ((78-283)) --------------------RSVISSIFLSLYAGFLATLLALLLGAPTGYILARF-------DFPGKRLVESIIDVPVVVPHTVAGIALLTVFGS-RGLIGEPLESYIQFRDALPGIVVAMLFVSMPYLANSAREGFKSVDPRLENAARSLGAPLWKAFFFVTLPLSARYLLIGSVMTWARAISEFGAVVILAYYPMVGPTLIYDRFISYGLSASRPIAVLLILVTLSIFLVIR---------

General information: TITO was launched using: RESULT: Template: 3D31.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 832 -98756 -118.70 -511.69 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain C : 0.66 3D Compatibility (PKB) : -118.70 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.358

(partial model without unconserved sides chains): PDB file : Tito_3D31.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3D31-query.scw PDB file : Tito_Scwrl_3D31.pdb: