Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTALVGGSVYMILVAKV--------PRFGAITTIGLV-------------------IALFFLGTKHGAGSFLPGIICGLLADGVAHLGKYKDKTKNFLSFIIFAFSTTGP----------ILLMWIAPK------AYMATLLARGKS------------------QEYIDRIMVAPNPGTVLLFIASIVIGALVGALI--GQALSKKFAQKI- 5KO9 Chain:A ((2-271)) CGRTSCHLPRDVLTRACAYQDRRGQQRLPEWRDPDKYCPSYNKSPQSNSPVLLSRLHFEKDADSSERIIAPMRWGLVPSWFKESDPSKLQFNTTNCRSDTVMEKRSFKVPLGKGRRCVVLADGFYEWQRCQGTNQRQPYFIYFPQIEKVWDNWRLLTMAGIFDCWEPPEGGDVLYSYTIITVDSCKGLSDIHHRMPAILDGEEAVSKWLDFGEVSTQEALKLIHPTENITFHAVSSVVNNSRNNTPECLAPVA

General information: TITO was launched using: RESULT: Template: 5KO9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 824 -58528 -71.03 -309.67 target 2D structure prediction score : 0.25 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -71.03 2D Compatibility (Sec. Struct. Predict.) : 0.25 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.116

(partial model without unconserved sides chains): PDB file : Tito_5KO9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5KO9-query.scw PDB file : Tito_Scwrl_5KO9.pdb: