Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVAQNTRGVQLIEHVSPQMLKAQLESIFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY-VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRAGRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVITAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD 4JJP Chain:B ((12-246)) -------LTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAITAQNTKGVFAVEDLNKKIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVENLKKYNPKYLVVDPVMI-----YLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMKRVGEEIL-QLGPKFVLMKGG-----AVDILVGKNIFKVYKS-----------GCTLSSAITSYLALGYEITEAVNLSKIYITEA---------------------

General information: TITO was launched using: RESULT: Template: 4JJP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1179 -74617 -63.29 -350.31 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -63.29 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.590

(partial model without unconserved sides chains): PDB file : Tito_4JJP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JJP-query.scw PDB file : Tito_Scwrl_4JJP.pdb: