Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFVARDARGELVNVLEDKLEKQAYTCPAC-----GGQLHLRQGPSVRTHFAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNLALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGFYVW----ELDKGKQVLRLKYLIYQDL--RGKLHYQIKEFSYGQGSLLEILRLPYKKQKISHFTVSEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIEQDLTSYYQHFYTYYQKNPQNDWQKL-YPPAFYQQYFLKNMVE------ 4ID9 Chain:A ((21-341)) MILVTGSAGRVGRAVVAALRTQGRTVRGFDLRPSGTGGEEVVGSLEDGQALSDAIMGVSAVLHLGAFMSWAPADRDRMFAVNVEGTRRLLDAASAAGVRRFVFASSGEVYPENRPEFLPVTEDHPLCPNSPYGLTKLLGEELVRFHQRSGAMETVILRFSHTQDATELLDEDSFFSGPRFFLRPRIHQQQNFGNAAIAELLQSRDIGEPSHILARNENGRPFRM---HITDTRDMVAGILLALDHPEA-----AGGTFNLGAD-EPADFAALLPKIAALTGL-----PIVTVDFPGDGVYYHTSNERIRNTLGFEAEWTMDRMLEEAATARRQRL

General information: TITO was launched using: RESULT: Template: 4ID9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1648 -1426 -0.87 -4.71 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -0.87 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.144

(partial model without unconserved sides chains): PDB file : Tito_4ID9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ID9-query.scw PDB file : Tito_Scwrl_4ID9.pdb: