Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYMNYTPKVRQKKSNFWGVFIMKLTYDDKVQIYELRKQ-GYSLEKLSNKFGINNSNLRYMIKLIDR 2LVS Chain:A ((4-43)) -------------------------VNDSLDIVEKLYKDGVPVKEIAKRSNNSMSTVYKALEKLE-

General information: TITO was launched using: RESULT: Template: 2LVS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 102 7787 76.34 199.67 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 76.34 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.817

(partial model without unconserved sides chains): PDB file : Tito_2LVS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LVS-query.scw PDB file : Tito_Scwrl_2LVS.pdb: