Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKLLREARQELAQLLRTKPQHIFFTSGGTEANNTTIIGYCLRHQEQGKHIITTAIEHHAVLETIDYLVQHFGFEATIIQPENQEITA-QQIQKALRDDTILVSTMFANNETGNLLPIAEIGQILKQ--HPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLY-ASSMDFDSYLHGGDQEQKKRAGTENLPAIVGMVAALKENLEKQEEHFQHVQNLETAFLAELEGIQYYLNRGKH-------HLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMYGANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG 1ECX Chain:B ((3-373)) -VYFDNNATTRVDDRVLEEMIVFYREKYGNPNSAHGMGIEANLHMEKAREKVAKVLGVSPSEIFFTSCATESINWILKTVAETFEKRKRTIITTPIEHKAVLETMKYLSMK-GFKVKYVPVDSRGVVKLEELEKLVDEDTFLVSIMAANNEVGTIQPVEDVTRIVKKKNKETLVHVDAVQTIGKIPFSLEKLEVDYASFSAHKFHGPKGVGITYIRKGVPIRPLIHGGGQERGLRSGTQNVPGIVGAARAMEIAVEELSEAAKHMEKLRSKLVSGL------MNLGAHIITPLEISLPNTLSVSFPNIRGSTLQNLLSGYGIYVST-----------RHVLDAM-GVDRRIAQGAIRISLCKYNTEEEVDYFLKKIEEIL--

General information: TITO was launched using: RESULT: Template: 1ECX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1992 -54183 -27.20 -155.25 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -27.20 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.544

(partial model without unconserved sides chains): PDB file : Tito_1ECX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ECX-query.scw PDB file : Tito_Scwrl_1ECX.pdb: