Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQSYSVIVVRSQEKKDALYELVPQEA-IRQSAVFLLFVGDLNRAEKGARLHTDTFQ-PQGVEGLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYPVFGMALGVPNQTHDVKPRLPLDNVVFEEEYQEQSTEAIQAYDRVQADYAGARATTS----WSQRLAEQFGQAEPSSTRKNLEQKKLL 5HEI Chain:H ((1-240)) MNEAIRTIQDHRSIRQYTDEAVSDEHLDTIIQSAQSAASSINGQQVTIISVQDKEKKKKLSELAGNQAWIDQAPLFLIFCADFNRAKIAAELNDAPLGVTDGLESILVGATDAGISLEAATVAAESLGLGTVPIGGIRRKPLEVIELLDLPEYVFPVSGLVVGHPSDHSAKKPRLPQAAVHHRESYNHDLKSLIQDYDAEMAEYMKKRTNGADDRNWSQTVSAIYKTIYYPEVRAMLEKQ---

General information: TITO was launched using: RESULT: Template: 5HEI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 957 -29441 -30.76 -125.81 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain H : 0.80 3D Compatibility (PKB) : -30.76 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_5HEI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HEI-query.scw PDB file : Tito_Scwrl_5HEI.pdb: