Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIFATSQVNPLTNSSVRNLRPKQRRLFMFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITAKLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLNTISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGLNDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL 1PES Chain:A ((327-337)) -------------------------------------------------------------------------------------------------------------------YFTLQIRGRER------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1PES.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1 -382 -381.50 -34.68 target 2D structure prediction score : 0.82 Monomeric hydrophicity matching model chain A : 0.36 3D Compatibility (PKB) : -381.50 2D Compatibility (Sec. Struct. Predict.) : 0.82 1D Compatibility (Hydrophobicity) : 0.36 QMean score : 0.868

(partial model without unconserved sides chains): PDB file : Tito_1PES.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PES-query.scw PDB file : Tito_Scwrl_1PES.pdb: