Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQIYLNGELTDTP-SQNLLQLIQELALE-------GKRFAVEHNQQIVPKSKLEQISIAQHDRIEIIHAVGGG 5MPO Chain:B ((44-106)) ---------ISVPQEIKALQLWKEIETRHPGLADVRNQIIFAVRQEYVELGDQ-LLVLQPGDEIAVIPPISGG

General information: TITO was launched using: RESULT: Template: 5MPO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 148 -2368 -16.00 -43.05 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.69 3D Compatibility (PKB) : -16.00 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.598

(partial model without unconserved sides chains): PDB file : Tito_5MPO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MPO-query.scw PDB file : Tito_Scwrl_5MPO.pdb: