Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MKMLAQLQNR--FDQWVEQIV------QYLDRLSVR--ERIMVVFTTIFVVVVIVGYSLWKMHSLAEQQQKRLNDLKDLMVWMQSNAVTMKPANELELDKSGKI--QRVAQQQGLTVSSQQNG----EQLQIVVTHQNYAILANFL----------IQLAQMGLSIQKMEMVSSEGQIKLTATV 1Y88 Chain:A ((16-199)) NLYFQGHMVARLLEEHGFETKTNVIVQGNCVEQEIDVVAERDGERYMIECKFHNIPV----YTGLKEAMYTYARFLDVEKHGFTQPWIFTNTKFSEEAKKYAGCVGIKLTGWSYPEKEGIEVLLESKGLYPITILRIDKEVLDELVRAGLVFCRDVVSAGEEKLREIGLSAKKAREVIAEAKKVIGGS-

General information: TITO was launched using: RESULT: Template: 1Y88.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 719 12946 18.00 84.61 target 2D structure prediction score : 0.27 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 18.00 2D Compatibility (Sec. Struct. Predict.) : 0.27 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.161

(partial model without unconserved sides chains): PDB file : Tito_1Y88.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1Y88-query.scw PDB file : Tito_Scwrl_1Y88.pdb: