Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFLSQIDEMLI-GD--LDLQFVMPWEFED-LNPEAFREFNRELYAHRFDLDYESYSVDNDIDLHLSNYYSSCETNKLIPSNC-FFKLVEYMLVVLESR-----SFTSLFSNCVEFYNTKYSNEYLTFF--LNLFSTSKSEVARDNLRMYITEIFDDKRYWANNELFDDSLLFEENLQQL--QIIRVM-- 2Q3F Chain:A ((3-181)) ----VKPRILLMGLRRSGKSSIQKVVFHKMSPNETLF------LESTNKICREDVSNSSFVNFQIWDFPGQIDFFDPTFDYEMIFRGTGALIFVIDSQDDYMEALARLHLTVTRAYKVNTDINFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEKIHLSFYLTSIYDHSIFEAFSKVVQKLIP

General information: TITO was launched using: RESULT: Template: 2Q3F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 698 6585 9.43 40.40 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 9.43 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.307

(partial model without unconserved sides chains): PDB file : Tito_2Q3F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Q3F-query.scw PDB file : Tito_Scwrl_2Q3F.pdb: