Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFVFNGFYTLISAVIVLLLGRFLVNRIDFLERYNIPEPVAGGLVAAVVSLLVHTFWGYSIVFSSELQTSFMLVF-FASIGLSANFMKLKEGGTALVIFLICVASFIVVQNAVGMSL-ATLL------GLDPLIGL--IAGSITLTGG--HGTAGAWGEILESQHGIQGALALGMASATFGLIM-CVIGGPLAKLLINRYSLAQAKTNAEIQQRDTHVEQNSDDLAPFENPHQVRLITADNAITTLGMFAACLAFAEFMTGFSKGTWFELPTFVWALGGGVILRNILESVLKVDIFDRAIDVFGNASLSLYLAMALLSLKLWQLADLAGPLVVILVAQTLTMALYAAFVTFRVMGKNYDAAVLAASHCGFGMGATPTAVANMQAITNMYGPSHKAFLIVPLCGAFFVDLINATVIQLMLKFIA 1ZCD Chain:A ((260-384)) -------------------------------------------------------------------AYLILPLFAFANAGVSLQGVTLDGLTSI--LPLGIIAGLLIGK-PLGISLFCWLALRLKLAHLPEGTTYQQIMV-VGILCGIGFTMSIFIASLAFG-SVDPELINWAKLGILVGSISSAVIGYSWLRVRL----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1ZCD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 324 -43997 -135.79 -392.83 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.49 3D Compatibility (PKB) : -135.79 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.49 QMean score : -0.003

(partial model without unconserved sides chains): PDB file : Tito_1ZCD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZCD-query.scw PDB file : Tito_Scwrl_1ZCD.pdb: