Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQDDISGWTEWNAHFEGIEEISSPSELHGLLTGIVCVTEAPTLEEWTQILTTLNVPELNEEALAL-LTDEAEDVAHALSEDELDYLPMLPDDEHLLQDRVQALSDWCAGVVLGFGLASGHVRTDERELIEHLQDVAAVEFEDSDNDEEGESSYEELYEFVRLIPVSLSIGRKKVTVAESSLLKNFYAKSKTSTVGTADQNIVEMFTPHRPS 2LXM Chain:A ((1-163)) --------------------MAALAPLPPL---------PAQFKSIQHHLRTAQEHDKRDPVVAYYCRLYAMQTGMKIDSKTPECRKFLSKLMDQLEALKKQLGD-------------NEAITQEIVGCAHLENYALKMFLYADNEDRAGRFHKNMIKSFYTASLLIDVITVFGELTDENVKHRKYARWKATYIHNCLKN------GETPQ

General information: TITO was launched using: RESULT: Template: 2LXM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 554 -15577 -28.12 -96.15 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -28.12 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.305

(partial model without unconserved sides chains): PDB file : Tito_2LXM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2LXM-query.scw PDB file : Tito_Scwrl_2LXM.pdb: