Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYVPTIENALVPVVVEQSSRGERSFDIYSRLLRERVIFLTGEVEDNMANLIVAQMLFLEAENPDKDIHLYINSPGGSVTAGMAIYDTMQFIKPDVVTYCMGQAASMGAFLLNAGAKGKRYCLENARVMIHQPLGGFRGQASDIEIHAREILFIKERLNRLMAEHSGQDYDTIARDTDRDNFMTAQAAKEYGLVDQVLSKRP 2FZS Chain:N ((2-192)) ---------LVPMV---------SFDIYSRLLKERVIFLTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVSTICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVKGRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHR-

General information: TITO was launched using: RESULT: Template: 2FZS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain N - contact count / total energy / energy per contact / energy per residue : 834 -133414 -159.97 -733.04 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain N : 0.91 3D Compatibility (PKB) : -159.97 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.91 QMean score : 0.567

(partial model without unconserved sides chains): PDB file : Tito_2FZS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FZS-query.scw PDB file : Tito_Scwrl_2FZS.pdb: