Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEHIERESMEFDVVIVGAGPAGLSAAIKIRQLAIENNLPDLSVCVVEKGSEVGAHILSGAVLEPHAINELFPNWKEEGAPLNVPVTEDKTFFLLSETTSKEAPHWMVPKT-MYNDGNYVISLGNVVRWLGQKAEELEVSIFPGFAASEVLYHENGTVKGIQTGDMGIGKDGEPTHNFTPGYELHAKYTLFAEGCRGHLGKRLIAKYNLDKDADPQHYGIGIKELWEIDPAKHKPGLVMHGAGWPLSETGSSGGWWLYHAENNQ--VTLGMIVDLSYENPHMYPFMEMQRWKTHPLIKQYLEGGKRISYGARAVVKGGFNSLPKLTFPGGCLIGDDAGFLNFAKIKGSHTAMKSGMLCGEAVFEAIAAGVEKGGDLAVARVTKGEDLFVKELTSYTEKFNNSWLKEELYNARNFGPAMHK-FGQWIGGAFNFIDQNVFK--VPFTLHDLKQDFAVLKTV-DATSFKPTYPKPDGKLTFDRLSSVFISNTVHEENQPAHLKLTDPSIPVNVNLPKWDEPAQRYCPAGVYEI--MENDDGSKRFQINAANCVHCKTCDIKDPSQNITWVTPEGGGGPNYPNM 2GMJ Chain:B ((28-584)) --NMERFAEEADVVIVGAGPAGLSAATRLKQLAAQHE-KDLRVCLVEKAAHIGAHTLSGACLDPRAFEELFPDWKEKGAPLNTPVTEDR-FGILTEKYRIPVP--ILPGLPMNNHGNYVVRLGHLVSWMGEQAEALGVEVYPGYAAAEILFHEDGSVKGIATNDVGIQKDGAPKTTFERGLELHAKVTIFAEGCHGHLAKQLYKKFDLRANCEPQTYGIGLKELWVIDEKKWKPGRVDHTVGWPL-DRHTYGGSFLYHLNEGEPLLALGFVVGLDYQNPYLSPFREFQRWKHHPSIKPTLEGGKRIAYGARALNEGGFQSIPKLTFPGGLLIGCSPGFMNVPKIKGTHTAMKSGTLAAESIFNQLTSENLQSKTIGL------------HVTEYEDNLKNSWVWKELYSVRNIRPSCHGILGVYGGMIYTGIFYWIFRGMEPWTLKHKGSDSDQLKPAKDCTPIE--YPKPDGQISFDLLSSVALSGTNHEHDQPAHLTLKDDSVPVNRNLSIYDGPEQRFCPAGVYEFVPLEQGDGF-RLQINAQNCVHCKTCDIKDPSQNINWVVPEGGGGPAYNGM

General information: TITO was launched using: RESULT: Template: 2GMJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 3362 -39780 -11.83 -72.59 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain B : 0.85 3D Compatibility (PKB) : -11.83 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_2GMJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2GMJ-query.scw PDB file : Tito_Scwrl_2GMJ.pdb: