Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIEKIKQAASFLYWGKMMAGFYRTNLGRVALQQRNIALNAKQRHLLLLID-HE-DFQTLNTEFKKR-IAPPELIQQLIDLKLIAPISENDSEFTEQIPLSESPTTSLEVKAQQKSTIDENESADLTGEIKVSLEPSCHSSNIENTQPPIPVQQLTFEEIQLLMKQSLSQYCGLMAKPLIQKIEQIKNLQELKMCQMQWITSLQESRIPPHELTHTLHSINYSIQLIQQKN 5JLU Chain:A ((36-91)) ----------------------------------DVKLTNTQEHILMLLSQQRLTNTDLAKALNISQAAVTKAIKSLVKQDMLAGTKDTV--------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5JLU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 153 -1780 -11.63 -33.58 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : -11.63 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.586

(partial model without unconserved sides chains): PDB file : Tito_5JLU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5JLU-query.scw PDB file : Tito_Scwrl_5JLU.pdb: