Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MTHTAVANVKLNWTYFCLILASTFLQGSSFVATKILLSSMSPLWVATIRF--FIAALSLLPLIIYRYFKNPISLFNIPWLKLFVIGL---FQTAGVMAFL-NIGLGYTSPSTAAILMASNPLLVVILAMLILGERISIRALVGLIVAFIGVVICIGLGN-----TNSGGIGRGEVLVILASSCWAIATIINKKFNLHLDPWVITF--------WQMLLGSLVL---------------------FLVALFSQQPFT--------LPTTESMWLTFLWLAIPAS---TGAMGLWFAALKIGGAIHTSGFLFLCPLFSAIITYFVLGTVLTSQELI------GGFLIGTG-IYVLSRYR----- 5TF6 Chain:A ((1-367)) MLTRNRELTTVLVKNLPKSYNQNKVYKYFKHCGPIIHVDVADSLKKNFRFARIEFARYDGALAAITKTHKVVGQNEIIVSHLTECTLWMTNFPPSYTQRNIRDLLQDINVVALSIRLPSLRFNTSRRFAYIDVTSKEDARYCVEKLNGLKIEGYTLVTKVSNPLEKSKRTDSATLEGREIMIRNLSTELLDENLLRESFEGFGSIEKINIPAGQKEHSFNNCCAFMVFENKDSAERALQMNRSLLGNREISVSLADKKPFLERNEVKRLLASRNSKELETLICLFPLSDKVSPSLICQFLQEEIHINEKDIRKILLVSDFNGAIIIFRDSKFAAKMLMILNGSQFQGKVIRSGTINDMKRYYNNQQN

General information: TITO was launched using: RESULT: Template: 5TF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1244 -100366 -80.68 -335.67 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -80.68 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.175

(partial model without unconserved sides chains): PDB file : Tito_5TF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5TF6-query.scw PDB file : Tito_Scwrl_5TF6.pdb: