Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MQEFLISTSIV----------ALAEMGDKTQLLALLLSACFRKPIPILIAILLATLINHGISAVLGQ-WITTVLSPAILVWVLAVGFIGMAFWMLIPDEL-DDETASINKWQKFGVFGATFILFFLAEIGDKT-QIATVALAARYDSIFWVM-----------LGTTL--GMMIANAPAVFIGNKLAERLS-IALIHKIGAAIFFIVG-------VSTLVQHYFF----------------------------------------------------------------------- 3G3L Chain:A ((10-305)) PAEAKYYIAGTITDATTGQELTTAKVTLGDKSV------TSSFNEQVNYKAEGYALVVSADGYYPVKRQVYLNQVSDGQTSVATVNVALVSVEAAVIPPVVPPTDPETDINEGEATKVADKAVEVAKPSESTVTDMLAGTTATPEEKKALDETLEMAGGMKVGETTPEVLADGSILAITPVKFT-NPIQDAPAMVPYFYNEGCELTGDVKEVAAPVAVAADIQKAFLSNAAKALNMNAGFVQKIGYTRISVLNGYSILGYTIKGQLVSKKLTFLISGKYYEGIVSYQKSVMIYPNYYS

General information: TITO was launched using: RESULT: Template: 3G3L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 630 -54598 -86.66 -296.73 target 2D structure prediction score : 0.23 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : -86.66 2D Compatibility (Sec. Struct. Predict.) : 0.23 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.057

(partial model without unconserved sides chains): PDB file : Tito_3G3L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G3L-query.scw PDB file : Tito_Scwrl_3G3L.pdb: