Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWQQLITWLKQLSFLVKKEFLTIFSDP----ANRAILFVPALMQALLSGYAATYDVNHVDYAILDQSNGQISHELISKLDGSGIFKRVATLEYTEQIKQVTDNRQALLIIAIPNDFESKLNNNQSAPIQVIVDGRN--SSTAMVAGSYLNKIIGQFNQQKFNSALPISLETRTWYNPNQESRWSLMPALIAALSMMQTLLLSALSVAREREQGTFDQLLVTPYTPLQIMIGKALPPIFVGLMQSTIILLIILFWFKIPMNGSIGLLYFGLFSFNVAVVGVGL--SISALSLNMQQA--MLFTFLLIMPLMLLSGLLTPVENMPKALQVATYANPLRFGINLVQRVYLEGASFAQVKLNFLPMIVLGIVTLPLAAWLFRNRLS 4TXD Chain:A ((21-369)) ----KRDVLAELGLGDLRPYLRSIGDKDRIFPKAHRIEVVFLVRAANYLLLRTEGAGTINMTTLEYSGGAMEVPVIQPQKLMAVTRRQMLQLLREYRDSLDEVSKRWLVQEVIGKAKDLGFKKVSEEWNCTIQGMDGFCPHCTIFGAALTE----QHNEKFGG-LSIGIKTRVRFDPAFATQRRITPET------HNKVTEGHLSM---TGQALFSEVHVEPGT---VFIGRAEL---VDLTEPELVATLYSLATLRELGGRSG--IYGTVRVEILGVKAGKYASTTAYDLAAENAGKGYEEVKKNLKERLEKLGFTPV-DNSKLLAAVDHKDPN----GLFKDLWRSSIDFAEKMVKWVEELKGGG---------------

General information: TITO was launched using: RESULT: Template: 4TXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1795 -57267 -31.90 -175.66 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -31.90 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.194

(partial model without unconserved sides chains): PDB file : Tito_4TXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TXD-query.scw PDB file : Tito_Scwrl_4TXD.pdb: