Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQHQINGIALPNEQGFFGEYGGQFIPPHLKEAMDEINVAYEEIRHTPEFQNELADLFANYVGRPSPLFHAKRLSEQLGGAQIYLKREDLNHTGAHKINHCLGEALLAKYMGKKKVIAETGAGQHGVALATACALVGIPCEIHMGQVDIEKEHPNVVKMKILGAHLVSVTRGTATLKDAVDSAFEEYLKDPKNFIYAIGSVVGPHPFPKMVRDFQSIIGKEIKEQTHQRFGKNPDYVVACVGGGSNAIGAFTAFLNDSDVKLVGVEPAGHGLDTNMHSATLTLGKPSQIHGMACYVLENEAGEPLPVHSIASGLDYPGVGPQHSFLKDLGRVEYSTATDQECLDAFMTLSRVEGIVPALESSHAVAWAIREAPKLPKETMIVVNLSGRGDKDSDYVAEKLKL 5EY5 Chain:D ((1-383)) --------------GRFGKYGGQYVPETLMPALEELEEAYERAKNDPEFQAELEYYLRDYVGRPTPLYFAENLTKDLGGAKIYLKREDLNHTGAHKINNALGQALLAKRMGKKRVIAETGAGQHGVATATVAAMFGLECVVYMGAEDIERQALNVFRMKLLGAKVRPVTSGSRTLKDAINEAMRDWVTNVEDTFYIIGSVVGPHPYPMMVRDFQSVIGEEARQQILEKEGRLPDAIVACVGGGSNAMGIFHPFIDDESVRLIGVEAAGKGIETGKHAATLSAGRPGVLHGAMTYLLQDEDGQIIEAHSISAGLDYPGVGPEHAYLKDTGRAEYVSVTDDEALEAFQLLSRTEGIIPALESSHAVAYAMKLAPELSKDQIIVVNLSGRGDKDVNTVAR----

General information: TITO was launched using: RESULT: Template: 5EY5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 2603 -180248 -69.25 -470.62 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain D : 0.88 3D Compatibility (PKB) : -69.25 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.573

(partial model without unconserved sides chains): PDB file : Tito_5EY5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EY5-query.scw PDB file : Tito_Scwrl_5EY5.pdb: