Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKQFAVIGNPIEQSRSPELHHAFAEKTGVDLNYQKRLAPLDGFESSMRSFFAEGGSGMNVTVPFKEQAFALCNVLTERAQIAKAVNTL-WMENGKLHGDNTDGQGLVAAIQALEWNLENTTILILGAGGATRGVIYPLVQAGAKKIVIANRTLARAEQLVDDLKTAVPQAQLQAISLNDLEGDFDIVINATSASLSGDALQLPEKLKF---KYAYEMAYGKPSSFLDQAKQRNVPYA-EGFGMLVGQAIEAFSIWNGVRPQLKDFL 3SEF Chain:C ((31-291)) ---QYAVFGNPINHSKSPFIHTLFARQTQQSMIYTAQCVPVDGFTEAAKHFFAQGGRGCNVTVPFKEEAYRFADRLTERARLAGAVNTLKK-DDGEILGDNTDGEGLVQDLLAQQVLLKGATILLIGAGGAARGVLKPLLDQQPASITVTNRTFAKAEQLA-ELVAAYGEVKAQA--FEQLKQSYDVIINSTS---------AIDPVIFSSRSVCYDMMYG-----------------IDGLGMLVGQAAESFMLWRGLRPGTKQIL

General information: TITO was launched using: RESULT: Template: 3SEF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1266 -47588 -37.59 -207.81 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain C : 0.82 3D Compatibility (PKB) : -37.59 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.515

(partial model without unconserved sides chains): PDB file : Tito_3SEF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SEF-query.scw PDB file : Tito_Scwrl_3SEF.pdb: