Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDITLKTPVEMRNVQLKVYDNTD--NSILIDT---QVASLTSYDIKLPSTKLNRLYRIEVTTQGSSQVFDPTKSEYQNISGVYHAFITPSSISNKTQLISPSSEAIYQRAVIRSGQLPNETIIPTRIEQLHVDLASQDVYRSLLNAFKDTNIPS-LSPASTLTILNLLKYSTTKPSTYVDSYLCFGYLQYWSSIHNTNSPYQDLTQSLATDLKDGYLDGKKLIGDKTSFTPIFTSAPDNIDPAKNTLLDIAANQKVTRDYFATNLRAATFKLADLQDQSHLDPIGYDLLDKKQYSGIIPAGNTSEFFRIDGAGDYRRAVGFSGITATCNGSAYPCKQGLTSINISDPKLPATDYLVGHYEDSTTGCQLNFRANGQIELIKGSQSYRSALSADSTDK---LLQTDAASYSYLLNSSSSEPNNSTQQYNFIQLHIKANKIVSAQAGLDSRKAPDVLQTTQLEYSFS------------------ 5DL8 Chain:A ((17-407)) --NPNQQTEDEWKFTLKNAYINRDFDNDALKDTGSWSQAASLFYKSKMHDTPLVIADKPITIGADASVQYAVRLSSDKHVAD------TVLPFNKETQ--SQASDYLKYGATLKLGY--DKTLLSVGELWLDLPVTAVDASRQLLTSYWGTNLKSQLSDQLYAEIGRVEKVSPRNE----EDFKKFSFTANGITKESDGLNYIDLRYQFTPSLKGEYYFGNL-----------------------EDLYNKHYVGLEHTWKQPTFALTSKFKYFNAKD------------DGNTFD--IDAENIGLLETVKV-KNHTFGLGYQQII---GESAYP---------LPDGFLPETYFI-------------NWNATGFFK--EDEKSYHVMYGYDFKDYIPGLNAMVKYVYGHDFKAANGEKNHETESNVILNYAFQ-----------QPLLKGFALQYIRIDYNVKHGNDFGEDRLFVNYTKKF

General information: TITO was launched using: RESULT: Template: 5DL8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1876 121023 64.51 332.48 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 64.51 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.049

(partial model without unconserved sides chains): PDB file : Tito_5DL8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DL8-query.scw PDB file : Tito_Scwrl_5DL8.pdb: