Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRLATRFEKLQSQQRKALVSYVMAGDPQPQVTVPLLHKMVAAGVDVIELGLPFSDPMADGPVIALAAERALAAGTNTLDALNMVKEFREQDQETPVVLMGYLNPVEVIGYEKFVSYAKQCGVDGLLLVDLPPEESKEFGAILKQHDMDQIFLLAPTSTDQRIQHVANQASGFIYYVSLKGVTGAATLDTSEAAARIEKIKGMTNVPVGVGFGISDAASAKAMGSVADAVIVGSAFVKSFATLAADE-AVEQTVNKVKELRAALDELV 5EY5 Chain:C ((2-261)) -NRIAEAFEELKKKGEKALIPFITAGDPDLETTLELVRALVEAGADIIELGIPFSDPLADGPTIQRASQRALASGTTLDKVFEMVRELREKNTDVPIVFLTYYNPIFRYGIERFVKECAEAGVDGLIVPDLPPEEAADLAAAAEKYGVDLIFLVAPTSTDERIKMIAKHASGFVYCVSVTGVT---------------RIRKHTDLPIAVGFGISTPEQAAEVAQVADGVIVGSAIVKRIEENQDEEDIVEEVREFVRELR-------

General information: TITO was launched using: RESULT: Template: 5EY5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1351 -184247 -136.38 -755.11 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain C : 0.83 3D Compatibility (PKB) : -136.38 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.648

(partial model without unconserved sides chains): PDB file : Tito_5EY5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EY5-query.scw PDB file : Tito_Scwrl_5EY5.pdb: