Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLQAVYLTPIELHQTPDWSDRPYNIHRDPLWKTEKQGKEFDDVWFYHCDHLGTPQEMTDHTGAIIWKAEYKAWGECKAEKAKSNFFENSEIISNNIRFQGQ-YFDE-ETGLHYNRYRYYSPYVGRFVSKDPIGLLGGSNNYAYAPSPTEWV-DPLGLSCQKIPKGFKSF-----GQLKQFGQAMQAGFSKLGFKGAT---MYMQGSAHSGRSFETGKAFDD--GRVSDFD---IAVVQPEL---FEKAKKMGIAKGNRTLPIEIN-SVEAN---KLGINGVLQKMSKLAGGR-DVNVMIFDSPESAKAKAEGTRIPANCM--------------------- 4UY5 Chain:A ((2-327)) --------TLSLANYLAADSAAEALRRDVRAGLTAAPKSLPPKWFYDAVGSDLFDQITRLPEYYPTRTEAQILRTRSAEIIAAAGADTLVELGSGTSEKTRMLLDAMRDA---ELLRRFIPFD---VDAGVLRSAGAAIGAEYPGIEIDAVCGDFEEHLGKIPHVGRRLVVFLGSTIGNLTPAPRAEFLSTLADTLQPGDSLLLGTDLVKDTGRLVRAYDDAAGVTAAFNRNVLAVVNRELSADFDLDAFEHVAKWNSD-EERIEMWLRARTAQHVRVAALDLEVDFAAGEEMLTEVSCKFRPENVVAELAEAGLRQTHWWTDPAGDFGLSLAVRLEHHHH

General information: TITO was launched using: RESULT: Template: 4UY5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1316 23616 17.94 84.04 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 17.94 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.114

(partial model without unconserved sides chains): PDB file : Tito_4UY5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UY5-query.scw PDB file : Tito_Scwrl_4UY5.pdb: