Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MARNADYIDI-AGFTIAPQTLLPYGHLYNASLAGNQLNSAHGFAGPILAAPIWLINKP-ERFFAIAPYLYVPIGTYHSDEALNIDENRWKFDLQLGVFNNVAMIFLPKSRPMLFDMVHMMILLE 4RL8 Chain:C ((52-151)) --RLLHVYQLTDRLTLEPQFLLPFGRVSSSGD-ASALGDTSGVGDLTLTAPLKYRLNEANDILGATVYLTAPTGNYNRDDALNLGENRWKVDLQAAYVKHLGE---------------------

General information: TITO was launched using: RESULT: Template: 4RL8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 353 -18168 -51.47 -185.38 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain C : 0.71 3D Compatibility (PKB) : -51.47 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.384

(partial model without unconserved sides chains): PDB file : Tito_4RL8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RL8-query.scw PDB file : Tito_Scwrl_4RL8.pdb: