Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSSLSQVNADVLKQAQQRIQQDLLTTLAAFSIPEPLKSAVHHAVMLGGKRVRPALCYATASLQDNPNFAAARRAAVAVELIHCYSLAHDDLPCMDNDLLRRGQPTCHVAFGEDTALLAGDILQSMAFEVLGSRLFDEQGQGTDAAIVLKQIQILATASSKMVCGQVLDLQAEAKQISQDELENIHRNKTGALIQAAIMMGAVTIFPGTDQAIPKLRQYGQAIGLAFQVQDDILDITSSTETLGKTAGKDEQVQKSTYPALMGLEQAQIYAKELHDQAFEALAHFGENAKELVEISQFLLARTN 4F62 Chain:B ((13-292)) ---------------QSRVDQYLEQQLSDYAPANQLHNAMRYSLF-GGKRIRPMLTYASAQLVGDIS-SLTDASAAALESIHAYSLIHDDLPAM---------------FDEATAILAGDALQTFAFELLS----NPTSAQPELAIKLIQELVVASGRNGMITGQMIDLSSEN--ISLAELEQMHVHKTGALIKASVRMGALSTGQVKPEQLAKLDAYAHAIGLAFQVQDDIIDL------------------KATYPKLLGLDGAKALVVRLHEQAIAQISEFGDKSQPLTDLANYIID---

General information: TITO was launched using: RESULT: Template: 4F62.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1153 -130082 -112.82 -533.12 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -112.82 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.534

(partial model without unconserved sides chains): PDB file : Tito_4F62.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4F62-query.scw PDB file : Tito_Scwrl_4F62.pdb: