Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSILVIADHNNQTLNGATLNVVAAAQ--KIGGDITVLVAGSGAQAVADAAA-KVAGVSKVLLADNAAY---ANQLAENVAGLVADLAKGYKYVLAASTTTGKNILPRVAALLDVSMITDIISVESANTFKRPIYAG-NAIATVQSD---EAIIVGTVRGTAFDPVAAEGGSAAVETVGEVKDAGV-SKFVSEEIVKLDRPELTAARIVVSGGRGVGSGENYHKVLDPLADKLGAAQGASRAAVDAGFVPNDFQVGQTGKIV-APELYIAVGISGAIQHLAGMKDSKVIVAINKDEEAPINSVADYWLVGDLNAVVPELVSKL 1O96 Chain:D ((3-318)) --ILVIAEHRRNDLRPVSLELIGAANGLKKSGEDKVVVAVIGSQADAFVPALSVNGVDELVVVKGSSIDFDPDVFEASVSALIA--AHNPSVVLLPHSVDSLGYASSLASKTGYGFATDVYIVEYQGDELVATRGGYNQKVNVEVDFPGKSTVVLTIRPSVFKPL--EGAGSPV--VSNVDAPSVQSRSQNKDYVEV-DIDITTVDFIMSIGRGIGEETNVEQFRE-LADEAGATLCCSRPIADAGWLPKSRQVGQSGKVVGSCKLYVAMGISGSIQHMAGMKHVPTIIAVNTDPGASIFTIAKYGIVADIFDIEEELKAQL

General information: TITO was launched using: RESULT: Template: 1O96.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1610 -242 -0.15 -0.81 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain D : 0.81 3D Compatibility (PKB) : -0.15 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.454

(partial model without unconserved sides chains): PDB file : Tito_1O96.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O96-query.scw PDB file : Tito_Scwrl_1O96.pdb: