Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTRPDSKSMNYQLLKTFSRQPIQFGRFLARLLAGLVNTLKITRTSKSIELNLRIALPYLTPQQRIAITEKAVRNELTSYFEFLSIWGSSNSKNISRIHRIEGEHFFHEALAAKKGVVLIVPHFGTWEVMNAWCAQF-------TSMTILYKPV--KNADADRFVREARSR--------EQANL-VPTD-----ESGVRQIFKALK-QGETTVILPDHTPNVGGD-MVNYFGVPLASSNLSA----KLIQKTKAK--ALFLYAIRNE----------------NDGFTIHIEPMDEKIYEGTADDGTYVIHQAIEQLIYQYPEHYHWSYKRFKANPALDNIYNIDPTEALKIVDRLKAEALKTSTQPEPIQTSVM 1K30 Chain:A ((127-313)) -------------------------------------------------------------------------------------------------------------LQQGHNVVLISNHQTEADPAIISLLLEKTNPYIAENTIFVAGDRVLADPLCK-PFSIGRNLICVYSKKHMFDIPELTETKRKANTRSLKEMALLLRGGSQLIWIAPSGGRDRPDPSTGEW--YPAPFDASSVDNMRRLIQHSDVPGHLFPLALLCHDIMPPPSQVEIEIRVIAFNGAGLSVAPEISF------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1K30.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 463 8449 18.25 61.67 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 18.25 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.364

(partial model without unconserved sides chains): PDB file : Tito_1K30.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1K30-query.scw PDB file : Tito_Scwrl_1K30.pdb: